Are you passionate about the enzymatic reactions of anaerobic microbes and keen to contribute to excellence in scientific research? Join us as a PhD student on a three-year fixed-term contract and be part of the IBS/IRIG Institute!

The EnLightEn project studies non-isolated archaea (derived from ‘microbial dark matter’) that produce and degrade methane, utilising native biomass rather than artificial systems.

Your tasks will include

• sampling and preparing microbial biomass (microbial enrichments cultured in flasks or bioreactors, as well as environmental samples),
• characterising the content of this biomass using microscopy and ‘omics’ techniques,
• to extract the proteins involved in catabolic processes and purify them using our oxygen-free process,
• to characterise them using biochemical, biophysical and structural tools,
• to analyse the data obtained to elucidate the reaction mechanisms,
  and to present these findings in the form of scientific articles.

This unconventional approach, which circumvents the barrier posed by microbial isolation, enables us to understand the molecular mechanisms of native enzymes involved in the conversion of carbon-based molecules, with potential applications in industrial chemistry. EnLightEn will open up a new field of research to characterise functional dark matter, which can then be applied to other metabolic processes and offer an unprecedented insight into the molecular tricks employed by the microbial world.

The ideal candidate is a microbiologist who is highly motivated to deepen their knowledge of protein biochemistry and structural biology in order to understand the fundamental enzymatic mechanisms of microbial metabolism. The candidate must hold a Master’s degree in microbiology and, preferably, have experience in environmental microbiology (e.g. microbial sampling). Candidates with strong skills and knowledge in anaerobic microbiology (e.g. anaerobic microbial culture, assays, metabolism, genome exploration, etc.) will be given priority. Experience in native protein purification, biochemistry and structural biology is welcome.

You will need to demonstrate an open mind and a high degree of scientific rigour. You must be fluent in English, which will enable you to communicate effectively with the team and write scientific articles.

🤝 Why join us? ….

By joining the Grenoble Interdisciplinary Institute, you’ll be part of a dynamic and innovative research environment where you’ll have the opportunity to learn, grow and play a key role within a centre of excellence.

…AND THAT’S NOT ALL!

You may be interested in the benefits that come alongside your main role:

A cutting-edge research ecosystem dedicated to topics of significant societal importance.
Training to enhance your skills and boost your career.
A staff committee offering a wealth of social, cultural and sporting benefits.
Practical benefits: staff canteen/meal vouchers, 85% of transport costs covered, and much more.
An exceptional living environment in Grenoble, nestled in the mountains, offering a unique balance between city life and nature.
🚀 Want to be part of the adventure? Apply now and put your energy to work for research and innovation!

In line with the CEA’s commitments to the integration of people with disabilities, this role is open to everyone. The CEA offers adjustments and/or organisational arrangements to facilitate the inclusion of disabled workers